Method for Determining 6 Psychotropic Drugs in Bovine Blood Using Anyeep TQ9100
This study utilized the Anyeep TQ9100 ultra-high-performance liquid chromatography-tandem mass spectrometry system from Anyeep to develop a method for simultaneously detecting 6 psychoactive drugs in bovine blood. The method demonstrated excellent linearity, with detection limits and quantification limits meeting relevant regulatory standards. Using this method and equipment enables reliable detection and quantification of psychoactive drugs.
Psychotropic drugs, including sedatives/hypnotics, antipsychotics, and central nervous system stimulants, pose health risks to humans through food chain contamination caused by their illegal use or addition in livestock farming and environmental residues. Accurate detection of these drugs is therefore critical. Among various sample types, bovine blood serves as a core matrix for monitoring psychotropic veterinary drug residues in beef cattle production, assessing exposure risks, and ensuring the safety of animal-derived foods.
Compared to urine and hair matrices, bovine blood features a complex composition with high protein content and low residues of psychotropic drugs, imposing stricter demands on the sensitivity, specificity, and sample preparation techniques of detection methods. Currently, detection of psychotropic drugs in bovine blood can be conducted in accordance with livestock and poultry veterinary drug residue regulations and GB/T national standards, while also referencing ISO 15189 and CLSI mass spectrometry standards. Precision qualitative and quantitative analysis is achieved using core technologies such as liquid chromatography-tandem mass spectrometry (LC-MS/MS). Implementing this testing approach plays a critical role in standardizing veterinary drug use in livestock farming, mitigating food safety risks, and safeguarding public health. This method is based on "Ministry of Agriculture and Rural Affairs Announcement No. 197-10-2019" and employsInQuantify by standard.
Instruments and Reagents
Anyeep TQ9100 Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry System
Acetonitrile(MS-grade, from Thermo Fisher Scientific, USA)
Formic acid (mass spectrometry grade, CNW Technologies, Germany)
Ammonium acetate (analytical grade, CNW Technologies, Germany)
Liquid Chromatography Conditions
Mobile Phase A: 0.1% Formic Acid/Water, containing 2 mM ammonium acetate
Mobile Phase B:Acetonitrile
Column: Phenomenex Kinetex F5 3.0 × 100 mm, 2.7 µm
Column Temperature: 40 °C
Flow rate: 0.4mL/min
Injection Volume:2μL
Mass Spectrometry Conditions
Ionization Source: Electrospray Ionization (ESI+)
Source Temperature: 550 ℃
Spray Voltage:5000 V
Curtain air: 20 psi
Mist60 psi
Auxiliary Air:60 psi
Collision Gas:4
Scan Mode: MRM
Sample Processing
Samples shall be processed in accordance with "Ministry of Agriculture and Rural Affairs Announcement No. 197-10-2019".
Summary
This study established an ultra-high-performance liquid chromatography-tandem mass spectrometry system, Anyeep TQ9100, for analysis.6A detection method for psychotropic drugs. Results demonstrate that this method exhibits excellent sensitivity, stability, and linear range, making it suitable for routine analysis and testing of psychotropic drugs.