Method for Determining Residues of 9 Penicillin Drugs Using Anyeep TQ9100
This study utilized the Anyeep TQ9100 ultra-high-performance liquid chromatography-tandem mass spectrometry system from Anyeep Company to establish a method for the simultaneous detection of nine penicillin drugs. The method demonstrated excellent linearity, with limits of detection and quantification meeting relevant standard requirements. Using this method and equipment enables the detection and quantification of penicillin drugs.
Penicillin is a highly effective, low-toxicity antibiotic with extensive clinical applications. Its development significantly enhanced humanity's ability to combat bacterial infections and spurred the emergence of the entire antibiotic family. Penicillin belongs to the β-lactam class of antibiotics. β-lactams are a group of antibiotics characterized by a four-membered lactam ring, including penicillins, cephalosporins, carbapenems, monobactams, and cephamycins. This class is widely used in clinical practice. These antibiotics offer strong bactericidal activity, low toxicity, broad indications, and excellent clinical efficacy.
Routine testing covers animal- and plant-derived foods, honey products, cosmetics, and other items. Applicable standards include GB/T 18932.25-2005, GB/T 20755-2006, GB/T 21315-2007, GB/T 22952-2008, GB/T 22975-2008, GB/T 37626-2019, SN/T 2534-2010, and others. This method is based on GB/T 22975-2008 and uses the external standard calibration for quantification.
Instruments and Reagents
Anyeep TQ9100 Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry System
Methanol (HPLC grade, Thermo Fisher Scientific, USA)
Water (Ultra-pure Water)
Formic acid (CNW, Germany)
Liquid Chromatography Conditions
Mobile PhaseA: 0.1% formic acid, water
Mobile Phase B: Methanol
Chromatography column：Waters ACQUITY UPLC HSS PFP 2.1×100 mm 1.8µm
Column Temperature：40 ℃
Flow rate: 0.3 mL/min
Injection volume：5 μL
Mass Spectrometry Conditions
Ionization Source： Electrospray Ionization Source (ESI)﹢）
Source Temperature： 5500 ℃
Spray Voltage： 5000 V
air curtain： 20 psi
Mist gas： 60 psi
Auxiliary Gas： 60 psi
Crash： 4
Scan Mode：MRM
Sample Processing
Samples are processed according to GB/T 22975-2008.
Summary
This study established an ultra-high-performance liquid chromatography-tandem mass spectrometry (Anyeep TQ9100) method for the analysis of nine penicillin antibiotics. Results demonstrate that the method offers excellent sensitivity, stability, and linear range, making it suitable for routine analysis of penicillin antibiotics.Test.