Determination of Nitrobenzene Compounds in Water Using the Anypu Single Quadrupole Gas Chromatography-Mass Spectrometry System
To protect the environment and ensure human health, national standards specify detection methods for nitrobenzene compounds in water. Referencing standard HJ 716-2014 "Determination of Nitrobenzene Compounds in Water—Gas Chromatography-Mass Spectrometry," this study developed a GC-MS-based method for detecting these compounds and validated it by evaluating linearity, repeatability, and limits of detection.
Instruments and Experimental Conditions
AnYiPu 7700
Injection volume: 1.0μL; Injection mode: splitless;
Injection Port: 280°C; Injection Port Purge Flow: 50mL/min; Purge Time: 0.75min
Column: HP-5MS 30m × 0.25mm × 0.25µm, Constant Flow Mode. Column Flow Rate: 1mL/min
Oven temperature program: 60°C (0 min) 10°C/min 200°C (0 min) 15°C/min 50°C (0 min)
Ion source: 280°C; Transfer line: 280°C; Solvent delay: 5 min;
Scan Mode: Full Scan (SCAN) and Selected Ion Monitoring (SIM); SCAN mode scan range: 40~500 amu.
Experimental Results
The chromatogram below shows the total ion current for 1 nitrobenzene standard compounds, with good separation of the 15 compounds.
Standard solutions of 15 nitrobenzene compounds were prepared at concentrations of 0.1mg/L, 0.5 mg/L, 1.0 mg/L, 2.0 mg/L, 5.0 mg/L, and 10.0 mg/L. Instrumental analysis yielded the linear equations for each compound as shown in Table 1. All linear correlation coefficients exceeded 0.99, indicating excellent linearity.
This study developed a method for detecting nitrobenzene compounds in water using gas chromatography-mass spectrometry (GC-MS). All linear correlation coefficients exceeded 0.99, relative standard deviations for repeatability ranged from 0.5% to 5.2%, and the method detection limits fully met relevant regulatory standards.